IMPACT OF POULTRY GROWTH STAGES ON TOXIC COMPOUNDS FORMATION DURING CHARCOAL GRILLING.
Keywords:
Poultry, Toxic Compounds, charcoal grilling, HPLC, GC-MS, polycyclic aromatic hydrocarbons (PAHs), heterocyclic amines (HCAs).Abstract
Poultry is a widely consumed source of protein, with commercial production supplying chickens of varying weights to meet market demands. However, high-temperature cooking methods, such as charcoal grilling, have been associated with the formation of toxic Compounds, including polycyclic aromatic hydrocarbons (PAHs) and heterocyclic amines (HCAs). This study aimed to evaluate the impact of grilling on toxic Compounds formation in poultry of different weight categories. Chicken breast samples were categorized into three groups (A1, A2, A2+ as un-grilled and B1, B2, B2+ as grilled) and analyzed using High-Performance Liquid Chromatography (HPLC) and Gas Chromatography-Mass Spectrometry (GC-MS).
Results showed a significant increase in chromatographic peak areas after grilling, indicating the formation of potential toxic Compounds. The highest percentage increase (554%) was observed in the lowest weight category (A1 to B1), whereas the lowest increase (66%) was seen in A2 to B2. This suggests that the chemical composition and growth stage of poultry influence the extent of toxic Compounds formation during grilling. GC-MS analysis further identified specific compounds related to these changes.
The findings highlight the potential health risks associated with grilling poultry, emphasizing the need for controlled cooking methods to minimize exposure to harmful toxic Compounds. Future research should focus on alternative cooking techniques and risk mitigation strategies to enhance food safety.
References
1. Ahmed, H. H., et al. (2016). Estimated daily intake and health risk of polycyclic aromatic hydrocarbons in grilled meat and chicken. International Journal of Current Microbiology and Applied Sciences, 5(2), 435-448. https://doi.org/10.20546/ijcmas.2016.502.048
2. Chung, S. Y., & Yettella, R. R. (2011). Effects of grilling and roasting on the levels of polycyclic aromatic hydrocarbons in beef and pork. Food Chemistry, 129(4), 1420-1426. https://doi.org/10.1016/j.foodchem.2011.05.086
3. Farhadian, A., Jinap, S., Hanifah, H. N., & Zaidul, I. S. (2011). Effects of marinating on the formation of polycyclic aromatic hydrocarbons (PAHs) in grilled meat. Food Control, 22(12), 2028-2033. https://doi.org/10.1016/j.foodcont.2011.05.025
4. Goliomytis, M., Panopoulou, E., & Rogdakis, E. (2003). Growth curves for body weight and major component parts, feed consumption, and mortality of male broiler chickens raised to maturity. Poultry Science, 82(7), 1061-1068. https://doi.org/10.1093/ps/82.7.1061
5. Hamzawy, A. H., et al. (2016). Estimated daily intake and health risk of polycyclic aromatic hydrocarbons in grilled meat and chicken. International Journal of Current Microbiology and Applied Sciences, 5(2), 435-448. https://doi.org/10.20546/ijcmas.2016.502.048
6. PubMed Central. (2020). The presence of polycyclic aromatic hydrocarbons (PAHs) in grilled meat: Risk assessment. Journal of Food Quality, 2020, Article ID 8872083. https://doi.org/10.1155/2020/8872083
7. PubMed. (2020). The presence of polycyclic aromatic hydrocarbons (PAHs) in grilled meat: Risk assessment. Journal of Food Quality, 2020, Article ID 8872083. https://doi.org/10.1155/2020/8872083
8. Wretling, S., Eriksson, A., Eskhult, G. A., & Larsson, B. (2010). Polycyclic aromatic hydrocarbons (PAHs) in Swedish smoked meat and fish. Journal of Food Composition and Analysis, 23(3), 264-272. https://doi.org/10.1016/j.jfca.2009.10.008
9. Zelinková, Z., & Wenzl, T. (2015). The occurrence of 16 EPA PAHs in food – A review. Polycyclic Aromatic Compounds, 35(2-4), 248-284. https://doi.org/10.1080/10406638.2014.918550
10. Healthline. (2021, June 22). Does charcoal cause cancer? Safety information about grilling. Retrieved from https://www.healthline.com/health/is-grilling-with-charcoal-or-other-heat-sources-carcinogenic
11. International Agency for Research on Cancer. (2010). Some non-heterocyclic polycyclic aromatic hydrocarbons and some related exposures. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, 92, 1-853. https://monographs.iarc.who.int/wp-content/uploads/2018/06/mono92.pdf
12. Kazerouni, N., Sinha, R., Hsu, C. H., Greenberg, A., & Rothman, N. (2001). Analysis of 200 food items for benzo[a]pyrene and estimation of its intake in an epidemiologic study. Food and Chemical Toxicology, 39(5), 423-436. https://doi.org/10.1016/S0278-6915(00)00158-2
13. National Cancer Institute. (2017, April 28). Chemicals in meat cooked at high temperatures and cancer risk. Retrieved from https://www.cancer.gov/about-cancer/causes-prevention/risk/diet/cooked-meats-fact-sheet
14. Pastor, A., & Makarski, B. (2013). The effect of dietary leucine and valine supplementation on growth performance and nitrogen balance in broiler chickens. Journal of Animal and Feed Sciences, 22(1), 56-62. https://doi.org/10.22358/jafs/65784/2013
15. Mac-Mod Analytical. (n.d.). Analysis of polycyclic aromatic hydrocarbons in grilled meat by UHPLC-MS-MS. Retrieved from https://www.mac-mod.com/wp-content/uploads/Analysis-of-Polycyclic-Aromatic-Hydrocarbons-in-Grilled-Meat-by-UHPLC-MS-MS.pdf
16. Khan, U., Jahangir, M., Nazir, M., Shakoor, A., Ahmad, E., Faizan Haider, H. M., ur-Rehman, A. (2025). A streamlined and authenticated GC-MS method for the simultaneous analysis of all regulated chlorinated organic carriers (COCs) from the consumer goods at ng mL−1 level. Journal of Liquid Chromatography & Related Technologies, 1–9. https://doi.org/10.1080/10826076.2025.2453530
17. Khan, U., & Jahangir, M. (2020). Optimisation and validation of a new gas chromatographic mass spectrometry method for the simultaneous analysis of all regulated flame retardants from consumer articles. International Journal of Environmental Analytical Chemistry, 102(2), 470–482. https://doi.org/10.1080/03067319.2020.1723568
18. Khan, U., & Jahangir, M. (2019). Development of a new gas chromatographic-mass spectrometry method for the simultaneous analysis of all regulated phthalates in consumer goods. International Journal of Environmental Analytical Chemistry, 100(11), 1299–1308. https://doi.org/10.1080/03067319.2019.1651850
19. Jahangir M, Khan U. Development of an Efficient Analytical Method for the Extraction and Analysis of Biocide Contents from the Textile Test Specimens on LC-DAD. J Anal Methods Chem. 2020 Mar 30; 2020: 3047961. doi: 10.1155/2020/3047961