IMPACT OF POULTRY GROWTH STAGES ON TOXIC COMPOUNDS FORMATIONDURING CHARCOAL GRILLING

Authors

  • Mohammad Tariq Qureshi Government College University, Faisalabad, Pakistan Author
  • Muhammad Shahid University of Gujrat, Gujrat, Pakistan Author
  • Mahnoor Fatima University of Management & Technology, Lahore Author
  • Samahir Khalid University of Engineering and Technology, Lahore, Pakistan Author
  • Ahmad Ali Khan North China University of Water Resources and Electric Power Zhengzhou, Henan Author
  • Khizar Hayat Government College University, Lahore, 54000, Pakistan Author
  • Razia Batool Government College University, Lahore, 54000, Pakistan Author
  • Madiha Batool Government College University, Lahore, 54000, Pakistan Author
  • Sabiha Naveed Government College University, Lahore, 54000, Pakistan Author
  • Ejaz Ahmad Government College University, Lahore, 54000, Pakistan Author
  • Abdul Shakoor Government College University, Lahore, 54000, Pakistan Author
  • Majid Nazir Government College University, Lahore, 54000, Pakistan Author
  • Uzman Khan Government College University, Lahore, 54000, Pakistan Author
  • Hafiz Muhammad Kashif Raza Department of Plant Breeding & Genetics, University of Agriculture, Faisalabad, Pakistan Author
  • Muhammad Abbas Raza Quraishi University of Agriculture, Faisalabad, Pakistan Author
  • Shamoon , Tariq Mohammad Ali Jinnah university Author

Keywords:

Poultry, Toxic Compounds, charcoal grilling, HPLC, GC-MS, polycyclic aromatic hydrocarbons (PAHs), heterocyclic amines (HCAs).

Abstract

Poultry is a widely consumed source of protein, with commercial production supplying chickens of varying weights to meet market demands. However, high-temperature cooking methods, such as charcoal grilling, have been associated with the formation of toxic Compounds, including polycyclic aromatic hydrocarbons (PAHs) and heterocyclic amines (HCAs). This study aimed to evaluate the impact of grilling on toxic Compounds formation in poultry of different weight categories. Chicken breast samples were categorized into three groups (A1, A2, A2+ as ungrilled and B1, B2, B2+ as grilled) and analyzed using High-Performance Liquid


Chromatography (HPLC) and Gas Chromatography-Mass Spectrometry (GC-MS). Results showed a significant increase in chromatographic peak areas after grilling, indicating the formation of potential toxic Compounds. The highest percentage increase (554%) was observed in the lowest weight category (A1 to B1), whereas the lowest increase (66%) was seen in A2 to
B2. This suggests that the chemical composition and growth stage of poultry influence the extent of toxic Compounds formation during grilling. GC-MS analysis further identified specific compounds related to these changes.


The findings highlight the potential health risks associated with grilling poultry, emphasizing the need for controlled cooking methods to minimize exposure to harmful toxic Compounds. Future research should focus on alternative cooking techniques and risk mitigation strategies to enhance food safety.

References

Ahmed, H. H., et al. (2016). Estimated daily intake and health risk of polycyclic aromatic hydrocarbons in grilled meat and chicken. International Journal of Current Microbiology and Applied Sciences, 5(2), 435-448. https://doi.org/10.20546/ijcmas.2016.502.048

Chung, S. Y., & Yettella, R. R. (2011). Effects of grilling and roasting on the levels of polycyclic aromatic hydrocarbons in beef and pork. Food Chemistry, 129(4), 1420-1426. https://doi.org/10.1016/j.foodchem.2011.05.086

Farhadian, A., Jinap, S., Hanifah, H. N., & Zaidul, I. S. (2011). Effects of marinating on the formation of polycyclic aromatic hydrocarbons (PAHs) in grilled meat. Food Control, 22(12), 2028-2033. https://doi.org/10.1016/j.foodcont.2011.05.025

Goliomytis, M., Panopoulou, E., & Rogdakis, E. (2003). Growth curves for body weight and major component parts, feed consumption, and mortality of male broiler chickens raised to maturity. Poultry Science, 82(7), 1061-1068.

https://doi.org/10.1093/ps/82.7.1061

Hamzawy, A. H., et al. (2016). Estimated daily intake and health risk of polycyclic aromatic hydrocarbons in grilled meat and chicken. International Journal of Current Microbiology and Applied Sciences, 5(2), 435-448.

https://doi.org/10.20546/ijcmas.2016.502.048

PubMed Central. (2020). The presence of polycyclic aromatic hydrocarbons (PAHs) in grilled meat: Risk assessment. Journal of Food Quality, 2020, Article ID 8872083. https://doi.org/10.1155/2020/8872083

PubMed. (2020). The presence of polycyclic aromatic hydrocarbons (PAHs) in grilled meat: Risk assessment. Journal of Food Quality, 2020, Article ID 8872083. https://doi.org/10.1155/2020/8872083

Wretling, S., Eriksson, A., Eskhult, G. A., & Larsson, B. (2010). Polycyclic aromatic hydrocarbons (PAHs) in Swedish smoked meat and fish. Journal of Food Composition and Analysis, 23(3), 264-272. https://doi.org/10.1016/j.jfca.2009.10.008

Zelinková, Z., & Wenzl, T. (2015). The occurrence of 16 EPA PAHs in food – A review.

Polycyclic Aromatic Compounds, 35(2-4), 248-284. https://doi.org/10.1080/10406638.2014.918550

Healthline. (2021, June 22). Does charcoal cause cancer? Safety information about grilling. Retrieved from https://www.healthline.com/health/is-grilling-with-charcoal-or- other-heat-sources-carcinogenic

International Agency for Research on Cancer. (2010). Some non-heterocyclic polycyclic aromatic hydrocarbons and some related exposures. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, 92, 1-853. https://monographs.iarc.who.int/wp- content/uploads/2018/06/mono92.pdf

Kazerouni, N., Sinha, R., Hsu, C. H., Greenberg, A., & Rothman, N. (2001). Analysis of 200 food items for benzo[a]pyrene and estimation of its intake in an epidemiologic study.

Food and Chemical Toxicology, 39(5), 423-436. https://doi.org/10.1016/S0278- 6915(00)00158-2

National Cancer Institute. (2017, April 28). Chemicals in meat cooked at high temperatures and cancer risk. Retrieved from https://www.cancer.gov/about- cancer/causes-prevention/risk/diet/cooked-meats-fact-sheet

Pastor, A., & Makarski, B. (2013). The effect of dietary leucine and valine supplementation on growth performance and nitrogen balance in broiler chickens. Journal of Animal and Feed Sciences, 22(1), 56-62.

https://doi.org/10.22358/jafs/65784/2013

Mac-Mod Analytical. (n.d.). Analysis of polycyclic aromatic hydrocarbons in grilled meat by UHPLC-MS-MS. Retrieved from https://www.mac-mod.com/wp- content/uploads/Analysis-of-Polycyclic-Aromatic-Hydrocarbons-in-Grilled-Meat-by- UHPLC-MS-MS.pdf

Khan, U., Jahangir, M., Nazir, M., Shakoor, A., Ahmad, E., Faizan Haider, H. M., ur- Rehman, A. (2025). A streamlined and authenticated GC-MS method for the simultaneous analysis of all regulated chlorinated organic carriers (COCs) from the consumer goods at ng mL−1 level. Journal of Liquid Chromatography & Related Technologies, 1–9. https://doi.org/10.1080/10826076.2025.2453530

Khan, U., & Jahangir, M. (2020). Optimisation and validation of a new gas chromatographic mass spectrometry method for the simultaneous analysis of all regulated flame retardants from consumer articles. International Journal of Environmental Analytical Chemistry, 102(2), 470–482. https://doi.org/10.1080/03067319.2020.1723568

Khan, U., & Jahangir, M. (2019). Development of a new gas chromatographic-mass spectrometry method for the simultaneous analysis of all regulated phthalates in consumer goods. International Journal of Environmental Analytical Chemistry, 100(11), 1299–1308. https://doi.org/10.1080/03067319.2019.1651850

Jahangir M, Khan U. Development of an Efficient Analytical Method for the Extraction and Analysis of Biocide Contents from the Textile Test Specimens on LC-DAD. J Anal Methods Chem. 2020 Mar 30; 2020: 3047961. doi: 10.1155/2020/3047961

Downloads

Published

2026-08-02

How to Cite

IMPACT OF POULTRY GROWTH STAGES ON TOXIC COMPOUNDS FORMATIONDURING CHARCOAL GRILLING. (2026). International Journal of NeuroOncology and Therapeutics, 1(1), 7-18. https://ijnot.com/index.php/ijnot/article/view/5

Similar Articles

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)

1 2 > >>