A NOVEL VALIDATED METHOD FOR EXTRACTION AND ESTIMATION OF DIMETHYL FUMARATE FROM VARIOUS TEXTILE MATRICES ON GC/MS.
Keywords:
Dimethyl fumarate (DMFu), Solvent extraction, Textile and Leather samples, GC/MS Analysis.Abstract
A novel and rapid analytical method was developed for the determination of extractable dimethyl fumarate (DMFu) in various materials, including leather, polyurethane (PU) leather, textiles, rubber, and silica gel pellets, using gas chromatography-mass spectrometry (GC/MS). DMFu was extracted using ethyl acetate and analyzed on a DB-624 capillary column with helium gas (99.9% purity) as the mobile phase. One target ion and two qualifier ions were selected for the identification and quantification of DMFu. Key pre-treatment parameters, such as extraction method, solvent, and time, were optimized to achieve optimal performance. Under the optimized conditions, the method demonstrated a recovery rate of 91% to 98% for the standard. The calibration curve exhibited excellent linearity with a regression value of 0.9995 and a working range of 0.01–0.50 mg L⁻¹. The relative standard deviation (RSD) was ≤10% for test specimens and ≤5% for the pure standard. The method's limit of detection (LOD) and limit of quantification (LOQ) were 5×10⁻⁴ mg L⁻¹ and 5×10⁻³ mg L⁻¹, respectively. The developed method was successfully applied to real samples, revealing DMFu concentrations ranging from 1.34 mg L⁻¹ to
5.12 mg L⁻¹. This approach provides a reliable and efficient means for the accurate determination of DMFu in diverse materials.
References
Wannick, M., Assmann, J. C., Vielhauer, J. F., Offermanns, S., Zillikens, D., Sadik, C. D., & Schwaninger, M. (2018). Frontiers in Immunology, 9, 1890. https://doi.org/10.3389/fimmu.2018.01890
Perini, P., Rinaldi, F., Puthenparampil, M., Marcon, M., Perini, F., & Gallo, P. (2018). Multiple Sclerosis and Related Disorders, 26,
https://doi.org/10.1016/j.msard.2018.08.014
Andersen, J. L., Gesser, B., Funder, E. D., Nielsen, C. J. F., Gotfred-Rasmussen, H., Rasmussen, M. K., Toth, R., Gothelf, K. V., Arthur, J. S. C., Iversen, L., & Nissen, P. (2018). Nature Communications, 9, 4344. https://doi.org/10.1038/s41467-018-06791-0
Ahmed, A. A., Ahmed, A. A. E., El Morsy, E. M., & Nofal, S. (2018). Journal of Pharmacy and Pharmacology, 70, 1521. https://doi.org/10.1111/jphp.12989
Ontaneda, D., Nicholas, J., Carraro, M., Zhou, J., Hou, Q., Babb, J., Riester, K., Mendoza, J. P., Livingston, T., & Jhaveri, M. (2018). Multiple Sclerosis and Related Disorders, 27,
https://doi.org/10.1016/j.msard.2018.10.004
Liu, L., Vollmer, M. K., Ahmad, A. S., Fernandez, V. M., Kim, H., & Doré, S. (2019). Free Radical Biology and Medicine, 131,
https://doi.org/10.1016/j.freeradbiomed.2018.11.031
Stefanelli, P., Girolimetti, S., Santilio, A., & Dommarco, R. (2011). Bulletin of Environmental Contamination and Toxicology, 86, 428. https://doi.org/10.1007/s00128-011- 0223-1
Amico, E. D., Zanghì, A., Callari, G., Borriello, G., Gallo, A., Graziano, G., Valentino, P., Buccafusca, M., Cottone, S., Salemi, G., Ragonese, P., Bossio, R. B., Docimo, R., Grimaldi,
L. M. E., Pozzilli, C., Tedeschi, G., Zappia, M., & Patti, F. (2018). Therapeutic Advances in Neurological Disorders, 11,
https://doi.org/10.1177/1756286418796404
Amico, E. D., Zanghì, A., Sciandra, M., Borriello, G., Callari, G., Gallo, A., Salemi, G., Cottone, S., Buccafusca, M., Valentino, P., Bossio, R. B., Grimaldi, L. M. E., Pozzilli, C., Tedeschi, G., Zappia, M., & Patti, F. (2018). Journal of Neurology, 266,
https://doi.org/10.1007/s00415-018-9155-6
Condé, S., Moisset, X., Pereira, B., Zuel, M., Colamarino, R., Maillet-Vioud, M., Lauxerois, M., Taithe, F., & Clavelou, P. (2018). European Journal of Neurology, 26,
https://doi.org/10.1111/ene.13844
Braune, S., Grimm, S., Van Hövell, P., Freudensprung, U., Pellegrini, F., Hyde, R., & Bergmann, A. (2018). Journal of Neurology, 265, 2980. https://doi.org/10.1007/s00415-018-
-9
Commission Regulation No. 412/2012. (2012). Official Journal of the European Union, 128, 1.
Abdelrahman, R. S., & Abdel-Rahman, N. (2019). Life Sciences, 217,
https://doi.org/10.1016/j.lfs.2018.12.019
Braley, T. J., Huber, A. K., Segal, B. M., Kaplish, N., Saban, R., Washnock-Schmid, J. M., & Chervin, R. D. (2018). Sleep, 41, 109. https://doi.org/10.1093/sleep/zsx154
Abd El-Fattah, A. A., Fahim, A. T., Sadik, N. A. H., & Ali, B. M. (2018). Brain Research, 1701, 227. https://doi.org/10.1016/j.brainres.2018.09.018
Singh, N., Saha, L., Kumari, P., Singh, J., Bhatia, A., Banerjee, D., & Chakrabarti, A. (2019). Brain Research Bulletin, 144,
https://doi.org/10.1016/j.brainresbull.2018.11.018
Høglund, R. A., Polak, J., Vartdal, F., Holmøy, T., & Lossius, A. (2018). Multiple Sclerosis and Related Disorders, 26, 90. https://doi.org/10.1016/j.msard.2018.09.012
Nachliely, M., Trachtenberg, A., Khalfin, B., Nalbandyan, K., Cohen-Lahav, M., Yasuda, K., Sakaki, T., Kutner, A., & Danilenko, M. (2018). Journal of Steroid Biochemistry and Molecular Biology, 188, 8. https://doi.org/10.1016/j.jsbmb.2018.11.013
Motterlini, R., Nikam, A., Manin, S., Ollivier, A., Wilson, J. L., Djouadi, S., Muchova, L., Martens, T., Rivard, M., & Foresti, R. (2019). Redox Biology, 20,
https://doi.org/10.1016/j.redox.2018.10.019
Lamas, J. P., Sanchez-Prado, L., Garcia-Jares, C., & Llompart, M. (2009). Journal of Chromatography A, 1216, 5755. https://doi.org/10.1016/j.chroma.2009.06.019
Lu, Y., & Zhu, Y. (2014). Talanta, 119, 430. https://doi.org/10.1016/j.talanta.2013.11.023
Narizzano, R., Risso, F., Venturelli, G., Devia, C., Carlini, E., & Maggiolo, S. (2009). Journal of Chromatography A, 1216,
https://doi.org/10.1016/j.chroma.2009.07.049
Stefanelli, P., Attard Barbini, D., Girolimetti, S., Santilio, A., & Dommarco, R. (2011). Journal of Environmental Science and Health, Part B, 46,
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